ges-1 cells Search Results


human gastric epithelium cell ges 1  (CLS Cell Lines Service GmbH)
94
CLS Cell Lines Service GmbH human gastric epithelium cell ges 1
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Human Gastric Epithelium Cell Ges 1, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pmc11849191-56-2-7?v=CLS+Cell+Lines+Service+GmbH
Average 94 stars, based on 1 article reviews
human gastric epithelium cell ges 1 - by Bioz Stars, 2026-07
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human normal gastricmucosa cell ges-1 line  (Biowit Technologies)
90
Biowit Technologies human normal gastricmucosa cell ges-1 line
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Human Normal Gastricmucosa Cell Ges 1 Line, supplied by Biowit Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pmc04297343-31-0-9?v=Biowit+Technologies
Average 90 stars, based on 1 article reviews
human normal gastricmucosa cell ges-1 line - by Bioz Stars, 2026-07
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immortalized human gastric epithelial cell line ges-1  (Biopharm GmbH)
90
Biopharm GmbH immortalized human gastric epithelial cell line ges-1
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Immortalized Human Gastric Epithelial Cell Line Ges 1, supplied by Biopharm GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pm26528757-158-9-18?v=Biopharm+GmbH
Average 90 stars, based on 1 article reviews
immortalized human gastric epithelial cell line ges-1 - by Bioz Stars, 2026-07
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ges-1 cells  (ScienCell)
90
ScienCell ges-1 cells
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Ges 1 Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pm36897043-110-2-7?v=ScienCell
Average 90 stars, based on 1 article reviews
ges-1 cells - by Bioz Stars, 2026-07
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ges-1 cells  (iCell Bioscience Inc)
90
iCell Bioscience Inc ges-1 cells
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Ges 1 Cells, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pm35873363-51-0-5?v=iCell+Bioscience+Inc
Average 90 stars, based on 1 article reviews
ges-1 cells - by Bioz Stars, 2026-07
90/100 stars
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ges-1 cell line  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection ges-1 cell line
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Ges 1 Cell Line, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/10__12659_slash_msm__913634-55-17-22?v=China+Center+for+Type+Culture+Collection
Average 90 stars, based on 1 article reviews
ges-1 cell line - by Bioz Stars, 2026-07
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ges1 cells  (BioResource International Inc)
90
BioResource International Inc ges1 cells
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Ges1 Cells, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pmc09057599__jci___132___155468___s209-28-5-14?v=BioResource+International+Inc
Average 90 stars, based on 1 article reviews
ges1 cells - by Bioz Stars, 2026-07
90/100 stars
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immortalized gastric mucosal epithelial cell line ges-1  (Beijing ComWin Biotech Co)
90
Beijing ComWin Biotech Co immortalized gastric mucosal epithelial cell line ges-1
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Immortalized Gastric Mucosal Epithelial Cell Line Ges 1, supplied by Beijing ComWin Biotech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pmc03948864-66-3-11?v=Beijing+ComWin+Biotech+Co
Average 90 stars, based on 1 article reviews
immortalized gastric mucosal epithelial cell line ges-1 - by Bioz Stars, 2026-07
90/100 stars
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human normal gastric epithelial cells ges-1  (HybriBio Limited)
90
HybriBio Limited human normal gastric epithelial cells ges-1
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Human Normal Gastric Epithelial Cells Ges 1, supplied by HybriBio Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pmc10008319-84-0-10?v=HybriBio+Limited
Average 90 stars, based on 1 article reviews
human normal gastric epithelial cells ges-1 - by Bioz Stars, 2026-07
90/100 stars
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normal gastric epithelial cell ges- 1  (VivaCell Biotechnology GmbH)
90
VivaCell Biotechnology GmbH normal gastric epithelial cell ges- 1
<t>Transplanted</t> <t>GES-1</t> gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01
Normal Gastric Epithelial Cell Ges 1, supplied by VivaCell Biotechnology GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pm40095756-49-9-22?v=VivaCell+Biotechnology+GmbH
Average 90 stars, based on 1 article reviews
normal gastric epithelial cell ges- 1 - by Bioz Stars, 2026-07
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human normal gastric epithelial cells (ges-1)  (BioVector NTCC)
90
BioVector NTCC human normal gastric epithelial cells (ges-1)
CARD11 is highly expressed in gastric cancer and is associated with poor prognosis. (A) The ENCORI database predicted the expression of CARD11 in tissues of patients with gastric cancer. *** P<0.001 vs. the cancer group. (B) The ENCORI database predicted the association of CARD11 overexpression with overall survival. Cutoff-High, median 50% and Cutoff-Low, median 50%. The expression levels of CARD11 in normal gastric <t>epithelial</t> cells and in gastric cancer cells were detected by (C) reverse transcription-quantitative PCR and (D) western blotting. ** P<0.01 and *** P<0.001 vs. the GES-1 group. CARD, caspase recruitment domain-containing protein; ENCORI, the encyclopedia of RNA interactomes.
Human Normal Gastric Epithelial Cells (Ges 1), supplied by BioVector NTCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pmc10433449-35-0-18?v=BioVector+NTCC
Average 90 stars, based on 1 article reviews
human normal gastric epithelial cells (ges-1) - by Bioz Stars, 2026-07
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ges-1 cell line  (Ribobio co)
90
Ribobio co ges-1 cell line
CARD11 is highly expressed in gastric cancer and is associated with poor prognosis. (A) The ENCORI database predicted the expression of CARD11 in tissues of patients with gastric cancer. *** P<0.001 vs. the cancer group. (B) The ENCORI database predicted the association of CARD11 overexpression with overall survival. Cutoff-High, median 50% and Cutoff-Low, median 50%. The expression levels of CARD11 in normal gastric <t>epithelial</t> cells and in gastric cancer cells were detected by (C) reverse transcription-quantitative PCR and (D) western blotting. ** P<0.01 and *** P<0.001 vs. the GES-1 group. CARD, caspase recruitment domain-containing protein; ENCORI, the encyclopedia of RNA interactomes.
Ges 1 Cell Line, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ges-1+cells/pmc07026158-33-0-25?v=Ribobio+co
Average 90 stars, based on 1 article reviews
ges-1 cell line - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


Transplanted GES-1 gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01

Journal: Stem Cell Research & Therapy

Article Title: Transplantation of gastric epithelial mitochondria into human gastric cancer cells inhibits tumor growth and enhances chemosensitivity by reducing cancer stemness and modulating gastric cancer metabolism

doi: 10.1186/s13287-025-04223-7

Figure Lengend Snippet: Transplanted GES-1 gastric epithelial mitochondria suppress gastric cancer cell stemness. ( A ) Protein expression of stemness markers (SOX2 and NANOG) after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of SOX2 and NANOG expression. ( C ) GRP78 expression after GES-1 mitochondrial transplantation for 24 h. ( D ) Quantification of GRP78 expression. ( E ) CD44 positive cells on MKN45 or AGS cells after GES-1 mitochondrial transplantation for 24 h. ( F ) Quantification of CD44 positive cells. ( G ) ALDH1 activity after mitochondrial transplantation for 24 h in MKN45 cells. ( H ) Quantification of ALDH1 activity. ( I ) CD24 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( J ) Quantification of CD24 positive cells. ( K ) LGR5 positive cells after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( L ) Quantification of LGR5 positive cells. ( M ) Expression of p-JNK and JNK after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( N ) NOTCH1 expression after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01

Article Snippet: The normal human gastric epithelium cell GES-1 (Cytion No. 305428) was obtained through Cytion (Cell Lines Service, Eppelheim, Germany), under a Material Transfer Agreement ( https://www.cytion.com/Our-Approach/Terms-and-Conditions ).

Techniques: Expressing, Transplantation Assay, Activity Assay, Two Tailed Test

Transplanted GES-1 gastric epithelial mitochondria into gastric cancer cells increased the level of intracellular ROS and modulated mitochondrial metabolism. ( A ) Superoxide generation in mitochondria was analyzed by performing mitoSOX Deep Red staining through flow cytometry after GES-1 mitochondrial transplantation for 24 h in MKN45 or AGS cells. ( B ) Quantification of mitochondrial superoxide generation. ( C ) Intracellular superoxide level was analyzed by performing DHE staining through flow cytometry after GES-1 mitochondrial transplantation for 24 h in MKN45 or AGS cells. ( D ) Quantification of intracellular superoxide level. ( E ) OXPHOS profiles, ( F ) spare respiratory capacity, ( G ) maximal respiration, and ( H ) basal respiration of MKN45 cells after GES-1 mitochondrial transplantation for 24 h were evaluated via a Seahorse bioanalyzer. ( I ) ATP concentration was analyzed via an ATPlite kit. Data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01

Journal: Stem Cell Research & Therapy

Article Title: Transplantation of gastric epithelial mitochondria into human gastric cancer cells inhibits tumor growth and enhances chemosensitivity by reducing cancer stemness and modulating gastric cancer metabolism

doi: 10.1186/s13287-025-04223-7

Figure Lengend Snippet: Transplanted GES-1 gastric epithelial mitochondria into gastric cancer cells increased the level of intracellular ROS and modulated mitochondrial metabolism. ( A ) Superoxide generation in mitochondria was analyzed by performing mitoSOX Deep Red staining through flow cytometry after GES-1 mitochondrial transplantation for 24 h in MKN45 or AGS cells. ( B ) Quantification of mitochondrial superoxide generation. ( C ) Intracellular superoxide level was analyzed by performing DHE staining through flow cytometry after GES-1 mitochondrial transplantation for 24 h in MKN45 or AGS cells. ( D ) Quantification of intracellular superoxide level. ( E ) OXPHOS profiles, ( F ) spare respiratory capacity, ( G ) maximal respiration, and ( H ) basal respiration of MKN45 cells after GES-1 mitochondrial transplantation for 24 h were evaluated via a Seahorse bioanalyzer. ( I ) ATP concentration was analyzed via an ATPlite kit. Data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and ** p < 0.01

Article Snippet: The normal human gastric epithelium cell GES-1 (Cytion No. 305428) was obtained through Cytion (Cell Lines Service, Eppelheim, Germany), under a Material Transfer Agreement ( https://www.cytion.com/Our-Approach/Terms-and-Conditions ).

Techniques: Staining, Flow Cytometry, Transplantation Assay, Concentration Assay, Two Tailed Test

Glycolytic and mitochondrial biogenesis proteins were downregulated after epithelial mitochondrial transplantation. ( A ) Protein expression of PKM2, MCT1, and MCT4 after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of PKM2, MCT1, and MCT4 expression after GES-1 mitochondrial transplantation for 24 h. ( C ) Lactate metabolism were analyzed using a Lactate-Glo Assay Kit after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( D ) Lactate secretion by MKN45 and GES-1 cells was analyzed using a Lactate-Glo Assay Kit. ( E ) Protein expression of PGC-1α after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( F ) Quantification of PGC-1α. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and *** p < 0.005

Journal: Stem Cell Research & Therapy

Article Title: Transplantation of gastric epithelial mitochondria into human gastric cancer cells inhibits tumor growth and enhances chemosensitivity by reducing cancer stemness and modulating gastric cancer metabolism

doi: 10.1186/s13287-025-04223-7

Figure Lengend Snippet: Glycolytic and mitochondrial biogenesis proteins were downregulated after epithelial mitochondrial transplantation. ( A ) Protein expression of PKM2, MCT1, and MCT4 after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( B ) Quantification of PKM2, MCT1, and MCT4 expression after GES-1 mitochondrial transplantation for 24 h. ( C ) Lactate metabolism were analyzed using a Lactate-Glo Assay Kit after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( D ) Lactate secretion by MKN45 and GES-1 cells was analyzed using a Lactate-Glo Assay Kit. ( E ) Protein expression of PGC-1α after GES-1 mitochondrial transplantation for 24 h in MKN45 cells. ( F ) Quantification of PGC-1α. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05 and *** p < 0.005

Article Snippet: The normal human gastric epithelium cell GES-1 (Cytion No. 305428) was obtained through Cytion (Cell Lines Service, Eppelheim, Germany), under a Material Transfer Agreement ( https://www.cytion.com/Our-Approach/Terms-and-Conditions ).

Techniques: Transplantation Assay, Expressing, Glo Assay, Two Tailed Test

Pretreatment of epithelial mitochondria into gastric cancer cells suppressed tumor growth in a mouse tumor xenograft model. ( A ) Experimental design and flowchart of the mouse xenograft model and mitochondrial treatment. AGS cells were pretreated with GES-1 mitochondria for 24 h before injection. ( B ) Schematic of the gastric cancer xenografts in each group. ( C ) Body weights of mice in each group. ( D ) Tumor growth curves of each group. ( E ) Gastric tumors harvested from each group. ( F ) Representative images of hematoxylin and eosin and IHC staining of Ki-67 and GRP78 in each group. ( G ). Representative IHC analysis of ki-67 staining in each group. ( H ) Representative IHC analysis of GRP78 in each group. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: ** p < 0.01 and *** p < 0.005

Journal: Stem Cell Research & Therapy

Article Title: Transplantation of gastric epithelial mitochondria into human gastric cancer cells inhibits tumor growth and enhances chemosensitivity by reducing cancer stemness and modulating gastric cancer metabolism

doi: 10.1186/s13287-025-04223-7

Figure Lengend Snippet: Pretreatment of epithelial mitochondria into gastric cancer cells suppressed tumor growth in a mouse tumor xenograft model. ( A ) Experimental design and flowchart of the mouse xenograft model and mitochondrial treatment. AGS cells were pretreated with GES-1 mitochondria for 24 h before injection. ( B ) Schematic of the gastric cancer xenografts in each group. ( C ) Body weights of mice in each group. ( D ) Tumor growth curves of each group. ( E ) Gastric tumors harvested from each group. ( F ) Representative images of hematoxylin and eosin and IHC staining of Ki-67 and GRP78 in each group. ( G ). Representative IHC analysis of ki-67 staining in each group. ( H ) Representative IHC analysis of GRP78 in each group. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: ** p < 0.01 and *** p < 0.005

Article Snippet: The normal human gastric epithelium cell GES-1 (Cytion No. 305428) was obtained through Cytion (Cell Lines Service, Eppelheim, Germany), under a Material Transfer Agreement ( https://www.cytion.com/Our-Approach/Terms-and-Conditions ).

Techniques: Injection, Immunohistochemistry, Staining, Two Tailed Test

Transplantation of gastric epithelial mitochondria promoted gastric cancer cell apoptosis under hypoxic conditions and enhanced chemosensitivity. ( A ) MKN45 or ( B ) AGS cells ( C ) were treated with CoCl 2 and GES-1 mitochondria for 48 h. Cell viability was analyzed via an SRB assay. Protein expression and quantification of HIF-1α in ( C ) MKN45 or ( D ) AGS cells were measured after GES-1 mitochondrial transplantation under hypoxic conditions for 24 h. ( E ) Protein expression and quantification of Drp1 in ( C ) MKN45 or ( D ) AGS cells were analyzed after GES-1 mitochondrial transplantation under hypoxic conditions. ( G ) Apoptotic protein expression was quantified on the basis of the c-csapase3/caspase3 ratio via Western blotting. ( H , I ) Caspase activity in AGS cells was analyzed through flow cytometry. ( J , K ) Caspase activity in MKN45 cells was analyzed through flow cytometry. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05, ** p < 0.01 and *** p < 0.001; # p < 0.05, ## p < 0.01 and ### p < 0.005

Journal: Stem Cell Research & Therapy

Article Title: Transplantation of gastric epithelial mitochondria into human gastric cancer cells inhibits tumor growth and enhances chemosensitivity by reducing cancer stemness and modulating gastric cancer metabolism

doi: 10.1186/s13287-025-04223-7

Figure Lengend Snippet: Transplantation of gastric epithelial mitochondria promoted gastric cancer cell apoptosis under hypoxic conditions and enhanced chemosensitivity. ( A ) MKN45 or ( B ) AGS cells ( C ) were treated with CoCl 2 and GES-1 mitochondria for 48 h. Cell viability was analyzed via an SRB assay. Protein expression and quantification of HIF-1α in ( C ) MKN45 or ( D ) AGS cells were measured after GES-1 mitochondrial transplantation under hypoxic conditions for 24 h. ( E ) Protein expression and quantification of Drp1 in ( C ) MKN45 or ( D ) AGS cells were analyzed after GES-1 mitochondrial transplantation under hypoxic conditions. ( G ) Apoptotic protein expression was quantified on the basis of the c-csapase3/caspase3 ratio via Western blotting. ( H , I ) Caspase activity in AGS cells was analyzed through flow cytometry. ( J , K ) Caspase activity in MKN45 cells was analyzed through flow cytometry. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: * p < 0.05, ** p < 0.01 and *** p < 0.001; # p < 0.05, ## p < 0.01 and ### p < 0.005

Article Snippet: The normal human gastric epithelium cell GES-1 (Cytion No. 305428) was obtained through Cytion (Cell Lines Service, Eppelheim, Germany), under a Material Transfer Agreement ( https://www.cytion.com/Our-Approach/Terms-and-Conditions ).

Techniques: Transplantation Assay, Sulforhodamine B Assay, Expressing, Western Blot, Activity Assay, Flow Cytometry, Two Tailed Test

Human gastric epithelial mitochondria reduce gastric cancer cell chemoresistance by promoting apoptosis. ( A , B ) MKN45 or AGS cells were treated with 5-FU or GES-1 mitochondria or both in combination for 24 h. Cell viability was analyzed via SRB Assay. ( C , D ) Protein expression and quantification of BAX in MKN45 or AGS cells were analyzed after treatment for 72 h. ( E – H ) Caspase activity in MKN45 or AGS cells was analyzed through flow cytometry. ( J ) Quantification of protein expression (p-AKT/AKT ratio) in MKN45 cells after GES-1 mitochondrial transplantation. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: ** p < 0.01 and *** p < 0.001; # p < 0.05, ## p < 0.01 and ### p < 0.005

Journal: Stem Cell Research & Therapy

Article Title: Transplantation of gastric epithelial mitochondria into human gastric cancer cells inhibits tumor growth and enhances chemosensitivity by reducing cancer stemness and modulating gastric cancer metabolism

doi: 10.1186/s13287-025-04223-7

Figure Lengend Snippet: Human gastric epithelial mitochondria reduce gastric cancer cell chemoresistance by promoting apoptosis. ( A , B ) MKN45 or AGS cells were treated with 5-FU or GES-1 mitochondria or both in combination for 24 h. Cell viability was analyzed via SRB Assay. ( C , D ) Protein expression and quantification of BAX in MKN45 or AGS cells were analyzed after treatment for 72 h. ( E – H ) Caspase activity in MKN45 or AGS cells was analyzed through flow cytometry. ( J ) Quantification of protein expression (p-AKT/AKT ratio) in MKN45 cells after GES-1 mitochondrial transplantation. The data are presented as the means ± SEMs; n ≥ 3 for independent experiments; two-tailed Student’s t test: ** p < 0.01 and *** p < 0.001; # p < 0.05, ## p < 0.01 and ### p < 0.005

Article Snippet: The normal human gastric epithelium cell GES-1 (Cytion No. 305428) was obtained through Cytion (Cell Lines Service, Eppelheim, Germany), under a Material Transfer Agreement ( https://www.cytion.com/Our-Approach/Terms-and-Conditions ).

Techniques: Sulforhodamine B Assay, Expressing, Activity Assay, Flow Cytometry, Transplantation Assay, Two Tailed Test

CARD11 is highly expressed in gastric cancer and is associated with poor prognosis. (A) The ENCORI database predicted the expression of CARD11 in tissues of patients with gastric cancer. *** P<0.001 vs. the cancer group. (B) The ENCORI database predicted the association of CARD11 overexpression with overall survival. Cutoff-High, median 50% and Cutoff-Low, median 50%. The expression levels of CARD11 in normal gastric epithelial cells and in gastric cancer cells were detected by (C) reverse transcription-quantitative PCR and (D) western blotting. ** P<0.01 and *** P<0.001 vs. the GES-1 group. CARD, caspase recruitment domain-containing protein; ENCORI, the encyclopedia of RNA interactomes.

Journal: Experimental and Therapeutic Medicine

Article Title: KLF5‑mediated expression of CARD11 promotes the progression of gastric cancer

doi: 10.3892/etm.2023.12121

Figure Lengend Snippet: CARD11 is highly expressed in gastric cancer and is associated with poor prognosis. (A) The ENCORI database predicted the expression of CARD11 in tissues of patients with gastric cancer. *** P<0.001 vs. the cancer group. (B) The ENCORI database predicted the association of CARD11 overexpression with overall survival. Cutoff-High, median 50% and Cutoff-Low, median 50%. The expression levels of CARD11 in normal gastric epithelial cells and in gastric cancer cells were detected by (C) reverse transcription-quantitative PCR and (D) western blotting. ** P<0.01 and *** P<0.001 vs. the GES-1 group. CARD, caspase recruitment domain-containing protein; ENCORI, the encyclopedia of RNA interactomes.

Article Snippet: Human normal gastric epithelial cells (GES-1) and human gastric cancer cells (MKN-45, KE-39 and AGS) were provided by BioVector NTCC Inc.

Techniques: Expressing, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot

Interference of CARD11 expression inhibits gastric cancer cell metastasis. The (A) migratory and (B) invasive abilities of gastric cancer cells transfected with si-CARD11 were detected by wound healing and Transwell assays. (C) The expression levels of EMT-related proteins in gastric cancer cells transfected with si-CARD11 were detected by western blot analysis. *** P<0.001 vs. the control group. ### P<0.001 vs. the si-NC group. CARD, caspase recruitment domain-containing protein; si, small interfering; EMT, epithelial-to-mesenchymal transition.

Journal: Experimental and Therapeutic Medicine

Article Title: KLF5‑mediated expression of CARD11 promotes the progression of gastric cancer

doi: 10.3892/etm.2023.12121

Figure Lengend Snippet: Interference of CARD11 expression inhibits gastric cancer cell metastasis. The (A) migratory and (B) invasive abilities of gastric cancer cells transfected with si-CARD11 were detected by wound healing and Transwell assays. (C) The expression levels of EMT-related proteins in gastric cancer cells transfected with si-CARD11 were detected by western blot analysis. *** P<0.001 vs. the control group. ### P<0.001 vs. the si-NC group. CARD, caspase recruitment domain-containing protein; si, small interfering; EMT, epithelial-to-mesenchymal transition.

Article Snippet: Human normal gastric epithelial cells (GES-1) and human gastric cancer cells (MKN-45, KE-39 and AGS) were provided by BioVector NTCC Inc.

Techniques: Expressing, Transfection, Western Blot, Control

KLF5 regulates CARD11 to promote malignant progression of gastric cancer might by activating mTOR pathway. The viability, proliferation and cell cycle of gastric cancer cells transfected with si-CARD11 and oe-KLF5 was analyzed by (A) CCK-8, (B and C) EdU staining and (D) flow cytometry analysis. The (E) migration and (F) invasion of gastric cancer cells transfected with si-CARD11 and oe-KLF5 was detected by wound healing and Transwell assays. (G) The expression of EMT-related proteins in gastric cancer cells transfected with si-CARD11 and oe-KLF5 was detected by western blotting. (H) The expression of mTOR pathway related proteins n gastric cancer cells transfected with si-CARD11 and oe-KLF5 was detected by western blotting. *** P<0.001 vs. Control group. # P<0.05, ## P<0.01 and ### P<0.001 vs. si-CARD11 group. KLF5, Krüppel-like factor 5; CARD, caspase recruitment domain-containing protein; si, small interfering; oe, overexpression; EMT, epithelial-to-mesenchymal transition; p-. phosphorylated.

Journal: Experimental and Therapeutic Medicine

Article Title: KLF5‑mediated expression of CARD11 promotes the progression of gastric cancer

doi: 10.3892/etm.2023.12121

Figure Lengend Snippet: KLF5 regulates CARD11 to promote malignant progression of gastric cancer might by activating mTOR pathway. The viability, proliferation and cell cycle of gastric cancer cells transfected with si-CARD11 and oe-KLF5 was analyzed by (A) CCK-8, (B and C) EdU staining and (D) flow cytometry analysis. The (E) migration and (F) invasion of gastric cancer cells transfected with si-CARD11 and oe-KLF5 was detected by wound healing and Transwell assays. (G) The expression of EMT-related proteins in gastric cancer cells transfected with si-CARD11 and oe-KLF5 was detected by western blotting. (H) The expression of mTOR pathway related proteins n gastric cancer cells transfected with si-CARD11 and oe-KLF5 was detected by western blotting. *** P<0.001 vs. Control group. # P<0.05, ## P<0.01 and ### P<0.001 vs. si-CARD11 group. KLF5, Krüppel-like factor 5; CARD, caspase recruitment domain-containing protein; si, small interfering; oe, overexpression; EMT, epithelial-to-mesenchymal transition; p-. phosphorylated.

Article Snippet: Human normal gastric epithelial cells (GES-1) and human gastric cancer cells (MKN-45, KE-39 and AGS) were provided by BioVector NTCC Inc.

Techniques: Transfection, CCK-8 Assay, Staining, Flow Cytometry, Migration, Expressing, Western Blot, Control, Over Expression